Lene Kleppe

Researcher

E-mail: lene.kleppe@hi.no

Telephone: 98620305

Department: Reproduksjon og utviklingsbiologi

Publications

Academic articles

2024

Loss of bmp15 function in the seasonal spawner Atlantic salmon results in ovulatory failure

Diego Crespo, Per Gunnar Fjelldal, Tom Johnny Hansen, Erik Nordtorp Kjærner-Semb, Kai Ove Skaftnesmo, Anders Thorsen, Birgitta Norberg, Rolf Brudvik Edvardsen, Eva Andersson, Rüdiger W. Schulz, Anna Wargelius, Lene Kleppe

The FASEB Journal 38

2024

Ocean warming shapes embryonic developmental prospects of the next generation in Atlantic cod

Kaja Helvik Skjærven, Maud Alix, Lene Kleppe, Jorge Manuel de Oliveira Fernandes, Paul Whatmore, Artem Nedoluzhko, Eva Andersson, Olav Sigurd Kjesbu

ICES Journal of Marine Science 81 p. 733-747

2024

Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.)

Eva Andersson, Rüdiger W. Schulz, Fernanda Almeida, Lene Kleppe, Kai Ove Skaftnesmo, Erik Nordtorp Kjærner-Semb, Diego Crespo, Per Gunnar Fjelldal, Tom Johnny Hansen, Birgitta Norberg, Rolf Brudvik Edvardsen, Anna Wargelius

Endocrinology 165

2022

Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut

Rolf Brudvik Edvardsen, Ola Wallerman, Tomasz Furmanek, Lene Kleppe, Patric Jern, Andreas Wallberg, Erik Nordtorp Kjærner-Semb, Stig Mæhle, Sara Olausson, Elisabeth Sundström, Torstein Harboe, Ragnfrid Mangor-Jensen, Margareth Møgster Drønen, Prescilla Perrichon, Birgitta Norberg, Carl-Johan Rubin

PLoS Genetics 18

2022

Pituitary Gonadotropin Gene Expression During Induced Onset of Postsmolt Maturation in Male Atlantic Salmon: In Vivo and Tissue Culture Studies

Diego Crespo, Kai Ove Skaftnesmo, Erik Nordtorp Kjærner-Semb, Ozlem Yilmaz, Birgitta Norberg, Sara Olausson, Petra Vogelsang, Jan Bogerd, Lene Kleppe, Rolf Brudvik Edvardsen, Eva Andersson, Anna Wargelius, Tom Johnny Hansen, Per Gunnar Fjelldal, Rüdiger W. Schulz

Frontiers in Endocrinology 13

2022

Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition

Lene Kleppe, Per Gunnar Fjelldal, Eva Andersson, Tom Johnny Hansen, Monica Sanden, Aina Bruvik, Kai Ove Skaftnesmo, Tomasz Furmanek, Erik Nordtorp Kjærner-Semb, Diego Crespo, Simon Flavell, Audun Østby Pedersen, Petra Vogelsang, Anja Torsvik, Karen Anita Kvestad, Sara Olausson, Birgitta Norberg, Rüdiger W. Schulz, J. Bogerd, N. Santi, Rolf Brudvik Edvardsen, Anna Wargelius

Aquaculture 560

2022

The Piwil1 N domain is required for germ cell survival in Atlantic salmon

Fernanda Loureiro Almeida, Kai Ove Skaftnesmo, Eva Andersson, Lene Kleppe, Rolf Brudvik Edvardsen, Birgitta Norberg, Per Gunnar Fjelldal, Tom Johnny Hansen, Rüdiger W. Schulz, Anna Wargelius

Frontiers in Cell and Developmental Biology 10

2021

Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar)

Kai Ove Skaftnesmo, Diego Crespo, Lene Kleppe, Eva Andersson, Rolf Brudvik Edvardsen, Birgitta Norberg, Per Gunnar Fjelldal, Tom Johnny Hansen, Rüdiger W. Schulz, Anna Wargelius

Frontiers in Cell and Developmental Biology 9

2020

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon

Hilal Guralp, Kai Ove Skaftnesmo, Erik Nordtorp Kjærner-Semb, Anne Hege Straume, Lene Kleppe, Rüdiger W. Schulz, Rolf Brudvik Edvardsen, Anna Wargelius

Scientific Reports 10

2020

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Kai Ove Skaftnesmo, Frida Thyri Segafredo, Anna Sofie Troedsson Wargelius

BMC Genomics 21 p. 1-15

2020

Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9- mediated HDR in Atlantic salmon

Anne Hege Straume, Erik Nordtorp Kjærner-Semb, Kai Ove Skaftnesmo, Hilal Guralp, Lene Kleppe, Anna Wargelius, Rolf Brudvik Edvardsen

Scientific Reports 10

2018

Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis

Erik Nordtorp Kjærner-Semb, Fernando Ayllon, Lene Kleppe, Elin Sørhus, Kai Ove Skaftnesmo, Tomasz Furmanek, Frida Thyri Segafredo, Anders Thorsen, Per Gunnar Fjelldal, Tom Johnny Hansen, Geir Lasse Taranger, Eva Andersson, Rüdiger W. Schulz, Anna Wargelius, Rolf Edvardsen

Scientific Reports 8:1912 p. 1-11

2018

Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis

Kjærner-Semb E, Ayllon F, Kleppe L, Sørhus E, Skaftnesmo KO, Furmanek T, Segafredo FT, Thorsen A, Fjelldal PG, Hansen T, Taranger GL, Andersson E, Schulz RW, Wargelius A, Edvardsen RB

Scientific Reports 8 p. 1912

2017

Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon

Kai Ove Skaftnesmo, Rolf Edvardsen, Tomasz Furmanek, D. Crespo, Eva Andersson, Lene Kleppe, Geir Lasse Taranger, Jan Bogerd, Rüdiger W. Schulz, Anna Wargelius

BMC Genomics 18

2017

Sex steroid production associated with puberty is absent in germ cellfree salmon

Lene Kleppe, Eva Andersson, Kai Ove Skaftnesmo, Rolf Edvardsen, Per Gunnar Fjelldal, Birgitta Norberg, Jan Bogerd, Rüdiger W. Schulz, Anna Wargelius

Scientific Reports 7

2017

Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon

Kai Ove Skaftnesmo, Rolf B. Edvardsen, Tomasz Furmanek, Diego Crespo, Eva Andersson, Lene Kleppe, Geir Lasse Taranger, Jan Bogerd, Rüdiger W. Schulz, Anna Wargelius

BMC Genomics 18 p. 801

2017

Sex steroid production associated with puberty is absent in germ cell-free salmon

Lene Kleppe, Kai Ove Skaftnesmo Eva Andersson, Rolf B. Edvardsen, Per Gunnar Fjelldal, Birgitta Norberg, Jan Bogerd, Rüdiger W. Schulz, Anna Wargelius

Scientific Reports 7 p. 12584

2017

Effects of dietary arachidonic acid on the reproductive physiology of female Atlantic cod (Gadus morhua L.)

Birgitta Norberg, Lene Kleppe, Eva Andersson, Anders Thorsen, Grethe Rosenlund, Kristin Hamre

General and Comparative Endocrinology 250 p. 21-35

2017

bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)

Lene Kleppe, Rolf Edvardsen, Tomasz Furmanek, Eva Andersson, Amélie Juanchich, Anna Wargelius

Molecular Reproduction and Development 84 p. 76-87

2016

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

Anna Wargelius, Sven Dirk Leininger, Kai Ove Skaftnesmo, Lene Kleppe, Eva Andersson, Geir Lasse Taranger, Rüdiger W. Schulz, Rolf Edvardsen

Scientific Reports 6

2016

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

Wargelius A, Leininger S, Skaftnesmo KO, Kleppe L, Andersson E, Taranger GL, Schulz RW, Edvardsen RB

Scientific Reports 6 p. 21284

2015

A comparison between egg trancriptomes of cod and salmon reveals species-specific traits in eggs for each species

Anna Wargelius, Tomasz Furmanek, Jérôme Montfort, Aurélie Le Cam, Lene Kleppe, Amélie Juanchich, Rolf Edvardsen, Geir Lasse Taranger, Julien Bobe

Molecular Reproduction and Development 82 p. 397-404

2015

Gonad specific genes in Atlantic salmon (Salmon salar L.): Characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes

Lene Kleppe, Anna Wargelius, Hanne Johnsen, Eva Andersson, Rolf Edvardsen

Gene 560 p. 217-225

2015

Gonad specific genes in Atlantic salmon (Salmo salar L.): characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes

Kleppe L, Wargelius A, Johnsen H, Andersson E, Edvardsen RB

Gene 560 p. 217-225

2015

A comparison between egg trancriptomes of cod and salmon reveals species-specific traits in eggs for each species

Wargelius A, Furmanek T, Montfort J, Le Cam A, Kleppe L, Juanchich A, Edvardsen RB, Taranger GL, Bobe J

Molecular Reproduction and Development 82 p. 397-404

2014

Global transcriptome analysis identifies regulated transcripts and pathways activated during oogenesis and early embryogenesis in atlantic cod

Lene Kleppe, Rolf Edvardsen, Tomasz Furmanek, Geir Lasse Taranger, Anna Wargelius

Molecular Reproduction and Development 81 p. 619-635

2014

Targeted mutagenesis in Atlantic salmon(Salmo salar L.)Using the CRISPR/Cas9 system induces Complete knockout individuals in the F0 generation

Rolf Edvardsen, Sven Dirk Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

PLOS ONE 9 p. 1-7

2014

Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod

Kleppe L, Edvardsen RB, Furmanek T, Taranger GL, Wargelius A

Molecular Reproduction and Development 81 p. 619-635

2014

Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation

Edvardsen RB, Leininger S, Kleppe L, Skaftnesmo KO, Wargelius A

PLoS One 9 p. e108622

2013

Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos

Lene Kleppe, Ørjan Karlsen, Rolf Edvardsen, Birgitta Norberg, Eva Andersson, Geir Lasse Taranger, Anna Wargelius

General and Comparative Endocrinology 189 p. 84-95

2013

Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos

Kleppe L, Karlsen Ø, Edvardsen RB, Norberg B, Andersson E, Taranger GL, Wargelius A

General and Comparative Endocrinology 189 p. 84-95

2012

Maternal 3 ' UTRs: from egg to onset of zygotic transcription in Atlantic cod

Lene Kleppe, Rolf Edvardsen, H Kuhl, Ketil Malde, Tomasz Furmanek, O Drivenes, R Reinhardt, Geir Lasse Taranger, Anna Wargelius

BMC Genomics 13

2012

Maternal 3’UTRs: from egg to onset of zygotic transcription in Atlantic cod

Kleppe L, Edvardsen RB, Kuhl, H, Malde K, Furmanek T, Drivenes Ø, Reinhardt R, Taranger GL, Wargelius A

BMC Genomics 13 p. 443

Other journal contributions

2021

Author Correction: Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon (Scientific Reports, (2020), 10, 1, (18042), 10.1038/s41598-020-74876-2)

Hilal Güralp, Kai Ove Skaftnesmo, Erik Nordtorp Kjærner-Semb, Anne Hege Straume, Lene Kleppe, Rüdiger W. Schulz, Rolf Brudvik Edvardsen, Anna Wargelius

Scientific Reports 11

Academic lectures

2016

Sterile salmon by targeting factors involved in germ cell survival: novel strategies for sustainable fish farming

Kleppe L, Edvardsen RB, Tveiten H, Leininger S, Skaftnesmo KO, Andersson E, Taranger GL, Scülz RW, Furmanek T, Wargelius A

2016

Targeting germ cell survival factors to produce sterile salmon through vaccination

Kleppe L, Wargelius A, Tveiten H, Leininger S, Skaftnesmo KO, Furmanek T, Schulz RW, Taranger GL, Andersson E, Edvardsen RB

2016

Targeting germ cell survival factors to produce sterile salmon

Kleppe L, Wargelius A, Tveiten H, Leininger S, Skaftnesmo KO, Furmanek T, Schulz RW, Taranger GL, Andersson E, Edvardsen RB

2013

Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod

Kleppe L, Edvardsen RB, Furmanek T, Taranger GL, Wargelius A

2011

Composition of maternal RNAs, from egg to onset of zygotic transcription in Atlantic cod

Kleppe L, Kuhl H, Malde K, Drivenes Ø, Edvardsen RB, Taranger GL, Reinhardt R, Wargelius A

2011

Torskegenomet: reproduksjonsstudier

Kleppe L

Scientific lectures

2024

Uses of biotechnology to control reproduction in Atlantic salmon

Lene Kleppe, Anna Sofie Troedsson Wargelius, Diego Crespo, Erik Nordtorp Kjærner-Semb, Kai Ove Skaftnesmo, Eva Andersson, Per Gunnar Fjelldal, Tom Johnny Hansen, Rolf Brudvik Edvardsen, Anders Thorsen, Rüdiger W. Schulz, Anne Torsvik, Grethe Beate Thorsheim, Anne Hege Straume, Petra Vogelsang, Ivar Helge Matre, Ann-Kathrin Jakobsen Kroken, Lise Dyrhovden, Audun Østby Pedersen, Simon Flavell, Florian Sambraus, Arnor Gullanger, Tone Knappskog

2024

Moving sterile Crispr salmon from the tank to sea cages - regulatory barriers and social impacts

Anna Sofie Troedsson Wargelius, Erik Nordtorp Kjærner-Semb, Anne Hege Straume, Per Gunnar Fjelldal, Jonatan Nilsson, Hilal Guralp, Kai Ove Skaftnesmo, Dorothy Jane Dankel, Eva Andersson, Lene Kleppe, Rolf Brudvik Edvardsen

2022

Atlantic cod facing climate-driven warming: reproductive physiology under challenging environmental conditions

Maud Alix, Lene Kleppe, Eva Andersson, Lena Birgitta Norberg, Ørjan Karlsen, Paul Whatmore, Jorge Manuel de Oliveira Fernandes, Artem Nedoluzhko, Kaja Helvik Skjærven, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2022

Atlantic cod facing climate-driven warming: Reproductive physiology under challenging environmental conditions

A. Maud, Lene Kleppe, Eva Andersson, Birgitta Norberg, Ørjan Karlsen, Paul Whatmore, Jorge Manuel de Oliveira Fernandes, Artem Nedoluzhko, Kaja Helvik Skjærven, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2019

How will ocean warming affect reproductive performance of Atlantic cod, Gadus morhua?

Maud Alix, Lene Kleppe, Eva Andersson, Lena Birgitta Norberg, Ørjan Karlsen, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2019

Highly efficient CRISPR/Cas9 knock-in in Atlantic salmon

Rolf Brudvik Edvardsen, Anne Hege Straume, Erik Nordtorp Kjærner-Semb, hilal guralp, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

2019

How will ocean warming affect the reproductive performance of Atlantic cod, Gadus morhua?

Maud Alix, Lene Kleppe, Eva Andersson, Birgitta Norberg, Ørjan Karlsen, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2018

Hvordan fungerer laksens pubertetsgen?

2018

Optimization of CRISPR/Cas9 knock-in technology and application in salmon

Rolf Edvardsen, Anne Hege Straume, Kai Ove Skaftnesmo, Lene Kleppe, hilal guralp, Anna Wargelius

2016

Targeting germ cell survival factors to produce sterile salmon

Lene Kleppe, Anna Wargelius, Helge Tveiten, Sven Dirk Leininger, Kai Ove Skaftnesmo, Tomasz Furmanek, Rüdiger W. Schulz, Geir Lasse Taranger, Eva Andersson, Rolf Edvardsen

2016

Targeting germ cell survival factors to produce sterile salmon through vaccination

Lene Kleppe, Anna Wargelius, Helge Tveiten, Sven Dirk Leininger, Kai Ove Skaftnesmo, Tomasz Furmanek, Rüdiger W. Schulz, Geir Lasse Taranger, Eva Andersson, Rolf Edvardsen

2016

Sterile salmon by targeting factors involved in germ cell survival: novel strategies for sustainable fish farming

Lene Kleppe, Rolf Edvardsen, Helge Tveiten, Sven Dirk Leininger, Kai Ove Skaftnesmo, Eva Andersson, Geir Lasse Taranger, Rüdiger W. Schulz, Tomasz Furmanek, Anna Wargelius

2016

Targeted mutagenesis in Atlantic salmon using the CRISPR/Cas9 system

Rolf Edvardsen, Lene Kleppe, Kai Ove Skaftnesmo, Sven Dirk Leininger, Tomasz Furmanek, Geir Lasse Taranger, Rüdiger W. Schulz, Eva Andersson, Anna Wargelius

2016

Bruk av CRISPR/Cas9 i laks

Rolf Edvardsen, Lene Kleppe, Kai Ove Skaftnesmo, Sven Dirk Leininger, Tomasz Furmanek, Geir Lasse Taranger, Rüdiger W. Schulz, Eva Andersson, Anna Wargelius

2016

Adaptive plasticity in Atlantic salmon- genetic predisposition of time at maturity and environmental triggers

Rolf Edvardsen, Erik Nordtorp Kjærner-Semb, Fernando Ayllon, Tomasz Furmanek, Tom Johnny Hansen, Per Gunnar Fjelldal, Carl Johan Rubin, Lene Kleppe, Kai Ove Skaftnesmo, Elin Sørhus, Faezeh Mohammadi, Geir Dahle, Vidar Wennevik, Monica Favnebøe Solberg, Kevin Glover, Birgitta Norberg, Geir Lasse Taranger, Eva Andersson, Rüdiger W. Schulz, Anna Wargelius

2014

A comparison between egg trancriptomes of cod and salmon reveals species specific traits in eggs for each species

Anna Wargelius, Tomasz Furmanek, Jerome Montfort, Aurelie Le Cam, Lene Kleppe, Rolf Edvardsen, Geir Lasse Taranger, Julien Bobe

2014

APPLICATIONS OF GENOME-WIDE TECHNIQUES TO STUDY SALMON PHYSIOLOGY, DEVELOPMENT AND REPRODUCTION

Geir Lasse Taranger, Rolf Edvardsen, Lene Kleppe, Eva Andersson, Anna Wargelius

2013

GLOBAL TRANSCRIPTOME ANALYSIS IDENTIFIES REGULATED TRANSCRIPTS AND PATHWAYS ACTIVATED DURING OOGENESIS AND EARLY EMBRYOGENESIS IN ATLANTIC COD

Lene Kleppe, Rolf Edvardsen, Tomasz Furmanek, Geir Lasse Taranger, Anna Wargelius

2011

Maternal 3'UTRs: from egg to onset of zygotic transcription in Atlantic cod

Lene Kleppe, Rolf Edvardsen, Heiner Kuhl, Ketil Malde, Tomasz Furmanek, Øyvind Drivenes, Richard Reinhardt, Geir Lasse Taranger, Anna Wargelius

Poster

2021

Long-term performance of gene edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition.

Lene Kleppe, Per Gunnar Fjelldal, Eva Andersson, Tom Johnny Hansen, Monica Sanden, Aina Bruvik, Kai Ove Skaftnesmo, Tomasz Furmanek, Erik Nordtorp Kjærner-Semb, Diego Crespo, Simon Flavell, Audun Ø. Pedersen, Petra Vogelsang, Anne Torsvik, Sara Olausson, Birgitta Norberg, Rüdiger W. Schulz, Jan Bogerd, Nina Santi, Rolf Brudvik Edvardsen, Anna Wargelius

2019

How climate change may affect reproductive performance of Atlantic cod

Maud Alix, Lene Kleppe, Eva Andersson, Lena Birgitta Norberg, Ørjan Karlsen, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2019

From oogenesis to embryonic development: How climate change may affect reproductive performance of Atlantic cod

Maud Alix, Lene Kleppe, Eva Andersson, Birgitta Norberg, Ørjan Karlsen, Anders Thorsen, Jon Egil Skjæraasen, Olav Sigurd Kjesbu

2018

VGLL3 AND THE HIPPO PATHWAY ARE REGULATED IN SERTOLI CELLS UPON ENTRY AND DURING PUBERTY IN ATLANTIC SALMON TESTIS.

2015

SCREENING FOR STERILITY CANDIDATE GENES IN ATLANTIC SALMON (SALMO SALAR)

Lene Kleppe, Rolf Edvardsen, Tomasz Furmanek, Eva Andersson, Anna Wargelius

2015

Screening for sterility candidate genes in Atlantic salmon (Salmo salar)

Kleppe L, Edvardsen RB, Furmanek T, Andersson, E, Wargelius A

2014

Candidate genes for sterility in Atlantic salmon (Salmo salar)

Lene Kleppe, Anna Wargelius, Eva Andersson, Geir Lasse Taranger, Rolf Edvardsen

2014

SMALL RNAS IN IMMATURE AND MATURE TESTIS OF ATLANTIC SALMON (SALMO SALAR L.)

Kai Ove Skaftnesmo, Rolf Edvardsen, Tomasz Furmanek, Eva Andersson, Lene Kleppe, Jan Bogerd, Rüdiger W. Schulz, Geir Lasse Taranger, Anna Wargelius

2014

Candidate genes for sterility in Atlantic salmon (Salmo salar)

Kleppe, L, Wargelius A, Andersson E, Taranger GL, Edvardsen RB

2011

The link between broodstock stress and offspring developmental potential in Atlantic cod Gadus morhua L

Lene Kleppe, Ørjan Karlsen, Rolf Edvardsen, Øyvind Drivenes, Birgitta Norberg, Geir Lasse Taranger, Anna Wargelius

2011

The link between broodstock stress and offspring developmental potential in Atlantic cod Gadus morhua L

Kleppe L, Karlsen Ø, Edvardsen RB, Norberg B, Andersson E, Taranger GL, Wargelius A

Reports and dissertations

2013

Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females

Kleppe, Lene

2013

Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females

Kleppe L

News

(18.03.2024) Cod can adjust to climate change - from one generation to the next

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