Lene Kleppe
Arbeidsområde
Reproduksjonsfysiologi og genetikk i oppdrettsarter som Atlantisk laks og Atlantisk torsk. Som masterstudent i akvakultur (Universitetet i Bergen (UiB)/Havforskningsinstituttet (HI) 2007-2009) studerte jeg effekter av fettsyreinnhold i fôr på genuttrykk i ovarier i torsk. Mitt doktorgradsarbeid (UiB/HI 2009-2013) hadde fokus på karakterisering av transkriptomet til ovarier under oogenese og i tidlige embryo, og beskrivelse av effekter av maternalt stress på eggkvalitet i torsk. Som postdoktor (HI 2013-2017) begynte jeg å arbeide med laks, nærmere bestemt identifisering, karakterisering og funksjonelle studier av kandidatgener som er involvert i dannelse av kjønnsceller og pubertet. I dag er jeg ansatt som forsker, og er involvert i prosjekter som hovedsakelig fokuserer på å forstå og finne løsninger på utfordringer knyttet til miljøeffekter (rømt laks) og fiskevelferd (tidlig pubertet) i lakseoppdrett.
Nøkkelord: Akvakultur, Atlantisk laks, Atlantisk torsk, reproduksjonsfysiologi, kjønnsceller, sterilitet, eggkvalitet, genetikk, genredigering.
Curriculum Vitae
UTDANNELSE
2009-2013: Doktorgrad, Universitetet i Bergen. Tittel på avhandlingen: "Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females".
2007-2009: Mastergrad i akvakultur, Universitetet i Bergen. Tittel på masteroppgaven: ”Quantification and localization of StAR in Atlantic cod ovaries”.
2003-2006: Bachelorgrad i biologi, Universitetet i Bergen.
ARBEIDSERFARING
2017-: Forsker, Havforskningsinstituttet, Bergen.
2013-2016: Postdoktor, Havforskningsinstituttet, Bergen.
2013: Senioringeniør, Havforskningsinstituttet, Bergen.
2009-2013: Stipendiat, Havforskningsinstituttet, Bergen.
2009: Forskningstekniker, Havforskningsinstituttet, Forskningsstasjon Matre, Matredal.
2008-2009: Røkter, Norsk Kveite AS, Askøy.
2006-2007: Forskningstekniker, Havforskningsinstituttet, Bergen.
PUBLIKASJONER
Kjærner-Semb, E, Ayllon, F, Kleppe, L, Sørhus, E, Skaftnesmo, KO, Furmanek, T, Segafredo, FT, Thorsen, A, Fjelldal, PG, Hansen, T, Taranger, GL, Andersson, E, Schulz, RW, Wargelius, A, Edvardsen, RB (2018). Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis, Scientific Reports 8:1912.
Skaftnesmo, KO, Edvardsen, RB, Furmanek, T, Crespo, D, Andersson, E, Kleppe, L, Taranger, GL, Bogerd, J, Schulz, RW, Wargelius, A (2017). Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon. BMC Genomics 18:801.
Kleppe, L, Andersson, E, Skaftnesmo, KO, Edvardsen, RB, Fjelldal, PG, Norberg, B, Bogerd, J, Schulz, RW, Wargelius, A (2017). Sex steroid production associated with puberty is absent in germ cell-free salmon. Scientific Reports 7:12584.
Norberg, B, Kleppe, L, Andersson, E, Thorsen, A, Rosenlund, G, Hamre, K (2017). Effects of dietary arachidonic acid on the reproductive physiology of female Atlantic cod (Gadus morhua L.). General and Comparative Endocrinology 250:21-35.
Kleppe L, Edvardsen RB, Furmanek T, Andersson E, Juanchich A, Wargelius A (2017). bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.). Molecular Reproduction and Development 84:76-87.
Wargelius A, Leininger S, Skaftnesmo KO, Kleppe L, Andersson E, Taranger GL, Schulz RW, Edvardsen RB (2016). Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon. Scientific Reports 6:21284.
Wargelius A, Furmanek T, Montfort J, Le Cam A, Kleppe L, Juanchich A, Edvardsen RB, Taranger GL, Bobe J (2015). A comparison between egg trancriptomes of cod and salmon reveals species-specific traits in eggs for each species. Molecular Reproduction and Development 82(5):397-404.
Kleppe L, Wargelius A, Johnsen H, Andersson E, Edvardsen RB (2015). Gonad specific genes in Atlantic salmon (Salmo salar L.): characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes. Gene 560(2):217-25.
Edvardsen RB, Leininger S, Kleppe L, Skaftnesmo KO, Wargelius A (2014). Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation. PLoS One 9(9):e108622.
Kleppe L, Edvardsen RB, Furmanek T, Taranger GL, Wargelius A (2014). Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod. Molecular Reproduction and Development 81(7):619-35.
Kleppe L, Karlsen Ø, Edvardsen RB, Norberg B, Andersson E, Taranger GL, Wargelius A (2013). Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos. General and Comparative Endocrinology 189:84-95.
Kleppe L, Edvardsen RB, Kuhl, H, Malde K, Furmanek T, Drivenes Ø, Reinhardt R, Taranger GL, Wargelius A (2012). Maternal 3’UTRs: from egg to onset of zygotic transcription in Atlantic cod. BMC Genomics 13: 443.
Doktorgradsavhandling, Februar 2013: Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females. Universitetet i Bergen.
Publikasjoner
Vitenskapelige artikler (NVI)
2024
Loss of bmp15 function in the seasonal spawner Atlantic salmon results in ovulatory failure
The FASEB Journal
38
2024
Ocean warming shapes embryonic developmental prospects of the next generation in Atlantic cod
ICES Journal of Marine Science
81
p. 733-747
2024
Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.)
Endocrinology
165
2022
Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
PLoS Genetics
18
2022
Pituitary Gonadotropin Gene Expression During Induced Onset of Postsmolt Maturation in Male Atlantic Salmon: In Vivo and Tissue Culture Studies
Frontiers in Endocrinology
13
2022
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition
Aquaculture
560
2022
The Piwil1 N domain is required for germ cell survival in Atlantic salmon
Frontiers in Cell and Developmental Biology
10
2021
Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar)
Frontiers in Cell and Developmental Biology
9
2020
Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
Scientific Reports
10
2020
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
BMC Genomics
21
p. 1-15
2020
Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9- mediated HDR in Atlantic salmon
Scientific Reports
10
2018
Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis
Scientific Reports
8:1912
p. 1-11
2018
Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis
Scientific Reports
8
p. 1912
2017
Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon
BMC Genomics
18
2017
Sex steroid production associated with puberty is absent in germ cellfree salmon
Scientific Reports
7
2017
Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon
BMC Genomics
18
p. 801
2017
Sex steroid production associated with puberty is absent in germ cell-free salmon
Scientific Reports
7
p. 12584
2017
Effects of dietary arachidonic acid on the reproductive physiology of female Atlantic cod (Gadus morhua L.)
General and Comparative Endocrinology
250
p. 21-35
2017
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)
Molecular Reproduction and Development
84
p. 76-87
2016
Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon
Scientific Reports
6
2016
Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon
Scientific Reports
6
p. 21284
2015
A comparison between egg trancriptomes of cod and salmon reveals species-specific traits in eggs for each species
Molecular Reproduction and Development
82
p. 397-404
2015
Gonad specific genes in Atlantic salmon (Salmon salar L.): Characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes
Gene
560
p. 217-225
2015
Gonad specific genes in Atlantic salmon (Salmo salar L.): characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes
Gene
560
p. 217-225
2015
A comparison between egg trancriptomes of cod and salmon reveals species-specific traits in eggs for each species
Molecular Reproduction and Development
82
p. 397-404
2014
Global transcriptome analysis identifies regulated transcripts and pathways activated during oogenesis and early embryogenesis in atlantic cod
Molecular Reproduction and Development
81
p. 619-635
2014
Targeted mutagenesis in Atlantic salmon(Salmo salar L.)Using the CRISPR/Cas9 system induces Complete knockout individuals in the F0 generation
PLOS ONE
9
p. 1-7
2014
Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod
Molecular Reproduction and Development
81
p. 619-635
2014
Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation
PLoS One
9
p. e108622
2013
Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos
General and Comparative Endocrinology
189
p. 84-95
2013
Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos
General and Comparative Endocrinology
189
p. 84-95
2012
Maternal 3 ' UTRs: from egg to onset of zygotic transcription in Atlantic cod
BMC Genomics
13
2012
Maternal 3’UTRs: from egg to onset of zygotic transcription in Atlantic cod
BMC Genomics
13
p. 443
Annet tidsskriftbidrag
2021
Author Correction: Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon (Scientific Reports, (2020), 10, 1, (18042), 10.1038/s41598-020-74876-2)
Scientific Reports
11
Vitenskapelige foredrag
2016
Sterile salmon by targeting factors involved in germ cell survival: novel strategies for sustainable fish farming
2016
Targeting germ cell survival factors to produce sterile salmon through vaccination
2016
Targeting germ cell survival factors to produce sterile salmon
2013
Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod
2011
Composition of maternal RNAs, from egg to onset of zygotic transcription in Atlantic cod
2011
Torskegenomet: reproduksjonsstudier
Faglige foredrag
2024
Uses of biotechnology to control reproduction in Atlantic salmon
2024
Moving sterile Crispr salmon from the tank to sea cages - regulatory barriers and social impacts
2022
Atlantic cod facing climate-driven warming: reproductive physiology under challenging environmental conditions
2022
Atlantic cod facing climate-driven warming: Reproductive physiology under challenging environmental conditions
2019
How will ocean warming affect reproductive performance of Atlantic cod, Gadus morhua?
2019
Highly efficient CRISPR/Cas9 knock-in in Atlantic salmon
2019
How will ocean warming affect the reproductive performance of Atlantic cod, Gadus morhua?
2018
Hvordan fungerer laksens pubertetsgen?
2018
Optimization of CRISPR/Cas9 knock-in technology and application in salmon
2016
Targeting germ cell survival factors to produce sterile salmon
2016
Targeting germ cell survival factors to produce sterile salmon through vaccination
2016
Sterile salmon by targeting factors involved in germ cell survival: novel strategies for sustainable fish farming
2016
Targeted mutagenesis in Atlantic salmon using the CRISPR/Cas9 system
2016
Bruk av CRISPR/Cas9 i laks
2016
Adaptive plasticity in Atlantic salmon- genetic predisposition of time at maturity and environmental triggers
2014
A comparison between egg trancriptomes of cod and salmon reveals species specific traits in eggs for each species
2014
APPLICATIONS OF GENOME-WIDE TECHNIQUES TO STUDY SALMON PHYSIOLOGY, DEVELOPMENT AND REPRODUCTION
2013
GLOBAL TRANSCRIPTOME ANALYSIS IDENTIFIES REGULATED TRANSCRIPTS AND PATHWAYS ACTIVATED DURING OOGENESIS AND EARLY EMBRYOGENESIS IN ATLANTIC COD
2011
Maternal 3'UTRs: from egg to onset of zygotic transcription in Atlantic cod
Poster
2021
Long-term performance of gene edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition.
2019
How climate change may affect reproductive performance of Atlantic cod
2019
From oogenesis to embryonic development: How climate change may affect reproductive performance of Atlantic cod
2018
VGLL3 AND THE HIPPO PATHWAY ARE REGULATED IN SERTOLI CELLS UPON ENTRY AND DURING PUBERTY IN ATLANTIC SALMON TESTIS.
2015
SCREENING FOR STERILITY CANDIDATE GENES IN ATLANTIC SALMON (SALMO SALAR)
2015
Screening for sterility candidate genes in Atlantic salmon (Salmo salar)
2014
Candidate genes for sterility in Atlantic salmon (Salmo salar)
2014
SMALL RNAS IN IMMATURE AND MATURE TESTIS OF ATLANTIC SALMON (SALMO SALAR L.)
2014
Candidate genes for sterility in Atlantic salmon (Salmo salar)
2011
The link between broodstock stress and offspring developmental potential in Atlantic cod Gadus morhua L
2011
The link between broodstock stress and offspring developmental potential in Atlantic cod Gadus morhua L
Rapporter og avhandlinger
2013
Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females
2013
Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females
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